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KMID : 0903619960370020324
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Journal of the Korean Society for Horticultural Science
1996 Volume.37 No. 2 p.324 ~ p.328
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Comparative Studies on the Hanabusaya asiatica and its Allied groups Based on Randomly amplified Polymorphic DNA ( RAPD) Analysis
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Abstract
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The phylogenetic relationships between Korean endemic Hanabusaya asiatica, and its allied groups including four genera and nine species were investigated at the DNA level using Randomly Amplified Polymorphic DNA (RAPD) method. Ten primers out of 80 primers (10-mer) screened gave rise to very high polymorphism (99%) in all of the tested plants, producing 153 randomly amplified DNA fragments. H. asiatica was differentiated from its allied groups at the 0.62 of similarity index of RAPDs. This results were in accordance with previous classification based on morphological studies. It was confirmed that H. asiatica could be placed into Korean endemic and suggested that RAPD technique be used as an additional method of phylogenetic relationship for plant systematics.
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KEYWORD
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